MicroRNA Regulation of Chronic Inflammation During Aging

Inclusion Criteria:

1. Age between 18 - 35y; 65y and older
2. BMI: \<30 kg/m2
3. Ability to sign informed consent
4. For older participant group: Clinical Frailty Scale score \< 3 and Mini-Cog score \> 3
5. Good general medical health, ambulatory and in independent living setting

Exclusion Criteria:

* History of cardiovascular disease (e.g., CHF, CAD, MI, CVA)
* History of endocrine or metabolic disease such as hypo/hyperthyroidism and diabetes (Treated hypo/hyperthyroid for at least 6 months will be permitted)
* History of kidney disease or failure (CKD \> stage 4)
* History of vascular disease
* Uncontrolled hypertension - Elevated systolic pressure \>150 or a diastolic blood pressure \> 100
* Cancer or history of successfully treated cancer (less than 1 year) other than basal cell carcinoma
* Chronic systemic corticosteroid use (≥ 2 weeks) within 4 weeks of enrollment and for study duration (intra-articular/topical/inhaled therapeutic or physiologic doses of corticosteroids will be permitted)
* Chronic anti-inflammatory medication use (≥ 2 weeks) within 4 weeks of enrollment
* Chronic inflammatory conditions (e.g., Rheumatoid Arthritis, Crohn's, fibromyalgia, lupus, colitis) HIV, Hepatitis B and C
* History of stroke with motor disability
* A recent history (\<12 months) of GI bleed
* History of liver disease or AST/ALT 2 times above the normal limit
* History of respiratory disease (acute upper respiratory infection, history of chronic lung disease)
* Current smokers or current tobacco use
* Current pregnancy as determined by positive pregnancy test
* Any staff members who report directly to the principal investigators.