Comparative Pharmacokinetics and Pharmacodynamics of Synthetic Nicotine

Inclusion Criteria:

* Healthy on the basis of medical history and limited physical examination.
* Current regular user of E-Cigarettes (≥ 15 days in the past 30 days)

Exclusion Criteria:

* • Medications

  * Use of medications that are inducers of nicotine metabolizing enzyme CYP2A6 (Example: rifampicin, carbamazepine, phenobarbital, and other anticonvulsant drugs).
  * Use of sympatholytic medications for cardiovascular conditions including hypertension (Example: beta and alpha-blockers).
  * Concurrent use of nicotine-containing medications (Example: nicotine patch, lozenge, gum).
  * Any stimulant medications (example: Adderall) generally given for ADHD treatment.

    • Pregnancy
  * Pregnancy (self-reported and urine pregnancy test)
  * Breastfeeding (determined by self-report)
  * Women of childbearing potential must be using an acceptable method of contraception

    * Inability to read and write in English
    * A known propylene glycol/vegetable glycerin allergy
    * Uncomfortable with getting blood drawn