How a Single Workout Affects Gut Bugs in Women With Different Fitness Levels and Body Types
The Acute Effects of an Endurance Exercise Bout the Gut Microbiome and Gut-derived Metabolome in Women's Distinct Fitness and Body Mass Indexes
Who can join
Ages 21 to 40 · Women · Healthy volunteers welcome
Full eligibility criteria
Inclusion Criteria: * Biological sex: female * BMI 19 to 24.99 kg/m2 or BMI 30-40 kg/m2 * Exercisers: Exercise at least 4 times per week for 60 minutes (a total of 240 minutes per week) on a programmed exercise training either on endurance sports (e.g., running, cycling, triathlon), strength (e.g., powerlifting), or team sports (e.g., rugby, football, soccer) * Non-Exercisers: Sedentary individuals who have not reached the PA guidelines (150 minutes of moderate-intensity physical activity a week, 75 minutes of vigorous-intensity physical activity, or an equivalent combination of moderate and vigorous-intensity physical activity). Exclusion Criteria: * Biological sex: male * BMI \< 19 kg/m2 or \> 40 kg/m2 * Currently pregnant, \< 2 years postpartum, lactating * Currently taking any herbal, fiber, or prebiotic supplement * Current or 1-month before the study, taking oral or vaginal antibiotics * Diagnosed with any gastrointestinal, endocrine, digestive, cancer, or cardiovascular disease.
About the study
This study aims to elucidate the differences in the gut microbiome functional activity and metabolome in adult premenopausal women with distinctive fitness levels and BMIs (with obesity, w/o obesity). The specific aims are as follows:
* Aim 1: To examine the effects of acute aerobic exercise at 60-70% heart rate reserve (HRRmax) for 30 minutes bout on changes in the abundance of SCFA-producing bacteria and their functional downstream metabolic activity. * Aim 2: To examine the effects of acute aerobic exercise at 60-70% HRRmax 30-minute bout on changes in GM-released SCFA concentrations in stool and plasmatic metabolome.
What is being tested
- Aerobic Exercise (behavioral)
Sponsor: George Washington University · Participants: 40 · Started: Oct 16, 2024
Contact the study team
- Carmen Ortega-Santos, PhD, RD · Phone: 2029942757
Official record on ClinicalTrials.gov — NCT06691100
Locations in the U.S.
| District of Columbia | Milken Institute School of Public Health, The George Washington University, Washington D.C. |
Conditions
From ClinicalTrials.gov, data retrieved Sep 30, 2026. Each study sets its own eligibility; the study team decides who can join.