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Dehydration, Rehydration, and Impact on Physiology

Impact of Mild Dehydration and Subsequent Rehydration on Cellular Stress and Physiological Performance

Who can join

Ages 45 to 65 · All sexes · Healthy volunteers welcome

Inclusion Criteria:

* Apparently Healthy
* BMI \< 30 kg/m2
* Body weight is at least 110 lbs

Exclusion Criteria:

* Currently pregnant
* Have Type 1 Diabetes or Type 2 Diabetes
* Have chronic kidney disease or a history of kidney stones
* Have hypertension
* Take medications that can cause fluid retention, such as corticosteroids, chronic use of Nonsteroidal Anti-inflammatory Drugs (NSAIDs), Hormonal Therapies (e.g., estrogen, androgens or anabolic steroids), Antihypertensives, Psychiatric Medications (e.g., Lithium), Vasodilators
* Take medications that reduce fluid retention: diuretics, and RAAS Inhibitors (e.g., ACE inhibitors and ARBs)

About the study

Our goal is to determine whether hydration status influences inflammation, and overall physiological performance in adults aged 45-65.

Aim 1: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on H2O2, upstream inflammatory signal (NFkB), mitochondrial fragmentation, and autophagy in circulating PBMCs.

Aim 2: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on fasting glucose, sleep performance, and strength performance.

Participant will:

complete a 4-day hydration protocol, followed by a 4-day dehydration protocol, and then a 4-day rehydration protocol.

attend five laboratory visits for testing: Day 1: Informed consent and familiarization Day 2: Pre-hydration testing (before the 4-day hydration protocol) Day 3: Pre-dehydration testing (before the dehyration protocol) Day 4: Post-dehydration testing (after the dehydration protocol) Day 5: Post-rehydration testing (after the rehydration protocol)

What is being tested

• Hydration-Dehydration-Rehydration Protocol (HDR) (behavioral)

Sponsor: Texas Tech University · Participants: 15 · Started: Jul 15, 2025

Contact the study team

• Hui Ying Luk, Ph.D. · Phone: 806-834-0827

Official record on ClinicalTrials.gov — NCT07084675

Locations in the U.S.

From ClinicalTrials.gov, data retrieved Oct 1, 2026. Each study sets its own eligibility; the study team decides who can join.