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Daily Upgrade Trial
TONE UP: Daily Upgrade Trial: Tone, Oxygenation, Neuromood, Energy - Upgrade Evaluation
Who can join
Ages 25 to 45 · Women · Healthy volunteers welcome
Full eligibility criteria
Inclusion Criteria: * Participant is an adult between the ages of 25-45 years * Participant has a recorded BMI between 18.5-30 kg/m\^2 * Participant is apparently healthy and free from disease, as determined by a health history questionnaire * Participant is a biological female * Participant is naturally menstruating or uses a form of hormonal contraceptive * Participant is recreationally active, based on the International Physical Activity Questionnaire- Short Form * Participant agrees to abstain from caffeine (12 hours), tobacco, alcohol, and exercise for 24 hours prior to testing days * Participant is willing and able to comply with the protocol Exclusion Criteria: * Participant has been diagnosed with chronic heart disease, arrhythmias, thyroid disease, renal, hepatic, autoimmune or neurological diseases, as determined by a health history questionnaire. * Participant has a musculoskeletal disorder or injury that would prohibit them from being able to safely complete the study protocol. * Participant is currently enrolled in a separate clinical trial or weight loss program involving diet, exercise, physical activity, or the potential ingestion of an active drug or dietary supplement * Participant is using, or has consistently used one of the following dietary supplements within 1 month prior to enrollment: whey protein, creatine, essential amino acids * Participant has a known allergy or sensitivity to the placebo or active ingredients * Participant is pregnant or planning to become pregnant * Participant does not speak English or is hearing impaired
About the study
This study evaluates the acute and chronic effects of an essential amino acid supplement (Daily Upgrade EAA+) on metabolomics, amino acid kinetics, metabolic rate, body composition in active adult women. The study consists of an acute randomized double-blind crossover phase comparing EAA+, whey protein isolate, and placebo before high-intensity interval training (HIIT), and a chronic randomized double-blind parallel-arm phase evaluating 3 weeks of EAA+ or placebo supplementation combined with twice-weekly HIIT.
What is being tested
- Essential Amino Acid+ (dietary supplement)
- Non-Calorie Placebo (dietary supplement)
- High intensity Interval Training (behavioral)
- No Exercise (other)
Sponsor: University of North Carolina, Chapel Hill · Participants: 44 · Started: Aug 31, 2026
Contact the study team
- Abbie Smith-Ryan, PhD · Phone: 919-962-2574
- Research Coordinator · Phone: 919-962-1663
Official record on ClinicalTrials.gov — NCT07792603
Locations in the U.S.
| North Carolina | Applied Physiology Laboratory, Chapel Hill |
From ClinicalTrials.gov, data retrieved Oct 1, 2026. Each study sets its own eligibility; the study team decides who can join.