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Daily Upgrade Trial

RecruitingHealthy volunteers welcome

TONE UP: Daily Upgrade Trial: Tone, Oxygenation, Neuromood, Energy - Upgrade Evaluation

Who can join

Ages 25 to 45 · Women · Healthy volunteers welcome

Full eligibility criteria
Inclusion Criteria:

* Participant is an adult between the ages of 25-45 years
* Participant has a recorded BMI between 18.5-30 kg/m\^2
* Participant is apparently healthy and free from disease, as determined by a health history questionnaire
* Participant is a biological female
* Participant is naturally menstruating or uses a form of hormonal contraceptive
* Participant is recreationally active, based on the International Physical Activity Questionnaire- Short Form
* Participant agrees to abstain from caffeine (12 hours), tobacco, alcohol, and exercise for 24 hours prior to testing days
* Participant is willing and able to comply with the protocol

Exclusion Criteria:

* Participant has been diagnosed with chronic heart disease, arrhythmias, thyroid disease, renal, hepatic, autoimmune or neurological diseases, as determined by a health history questionnaire.
* Participant has a musculoskeletal disorder or injury that would prohibit them from being able to safely complete the study protocol.
* Participant is currently enrolled in a separate clinical trial or weight loss program involving diet, exercise, physical activity, or the potential ingestion of an active drug or dietary supplement
* Participant is using, or has consistently used one of the following dietary supplements within 1 month prior to enrollment: whey protein, creatine, essential amino acids
* Participant has a known allergy or sensitivity to the placebo or active ingredients
* Participant is pregnant or planning to become pregnant
* Participant does not speak English or is hearing impaired

About the study

This study evaluates the acute and chronic effects of an essential amino acid supplement (Daily Upgrade EAA+) on metabolomics, amino acid kinetics, metabolic rate, body composition in active adult women. The study consists of an acute randomized double-blind crossover phase comparing EAA+, whey protein isolate, and placebo before high-intensity interval training (HIIT), and a chronic randomized double-blind parallel-arm phase evaluating 3 weeks of EAA+ or placebo supplementation combined with twice-weekly HIIT.

What is being tested

Sponsor: University of North Carolina, Chapel Hill · Participants: 44 · Started: Aug 31, 2026

Contact the study team

Official record on ClinicalTrials.gov — NCT07792603

Locations in the U.S.

North CarolinaApplied Physiology Laboratory, Chapel Hill

From ClinicalTrials.gov, data retrieved Oct 1, 2026. Each study sets its own eligibility; the study team decides who can join.