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Supplementation of Cholecalciferol and Related Active Metabolites Through Bioenhancing Layer's Eggs

Biofortified Vitamin D Metabolites in Eggs and Their Impact on Vitamin D Status and the Gut Microbiome

Who can join

Ages 18 to 40 · All sexes · Healthy volunteers welcome

Inclusion Criteria:

* Healthy adults
* Non-smoking
* Age between 18- 40 years
* Females: premenopausal and not pregnant or lactating
* Body mass index (BMI) between 18.5-27.0 kg/m2.
* No planned trip(s) to lower latitudes during the 8-week study

Exclusion Criteria:

* BMI \<18.5 or \> 27.0 kg/m2,
* Age \<18 y or \> 40y,
* Smoking
* Pregnancy, lactating
* Have gastrointestinal disorders/malabsorption diseases/dietary restrictions/use of medications known to impact vitamin D and 25(OH)D absorption
* Been prescribed antibiotics within 3 months before enrolling
* History or current diagnosis of any of the following conditions: intestinal problems (cystic fibrosis, Crohn's Disease, IBD, etc.), blood disorders (sickle cell, thalassemia, etc.), stomach problems (ulcers or other problems requiring antacids), joint diseases requiring anti-inflammatory medication, or asthma.
* Planned trip(s) to lower latitudes during the 8-week study

About the study

The purpose of this research is to evaluate if 1) consumption of vitamin D3- or 25(OH)D3-biofortified eggs will increase vitamin D and 25(OH)D status in young adults who consume these bioenhanced eggs, 2) to determine if the gut microbiome profile is associated with 25(OH)D or vitamin D status and 3) to evaluate possible changes in the gut microbiome following an 8-week supplementation period providing 5(OH)D- or vitamin D bioenhanced eggs.

What is being tested

• Vitamin D3 bioenhanced eggs (dietary supplement)
• 25(OH)D3 bioenhanced eggs (dietary supplement)

Sponsor: Cornell University · Participants: 60 · Started: 2027-01

Contact the study team

• Kimberly O'Brien, PhD · Phone: (607) 255-3743

Official record on ClinicalTrials.gov — NCT07859280

Locations in the U.S.

From ClinicalTrials.gov, data retrieved Oct 6, 2026. Each study sets its own eligibility; the study team decides who can join.