Intravital Microscopy in Human Solid Tumors

Inclusion Criteria:

* Age ≥ 18 years of age
* Eastern Cooperative Oncology Group (ECOG)Performance Status of ≤ 2
* Measurable tumor by direct visualization requiring surgical resection in the operating room (OR)
* Tumor types of origin include gastric, pancreatic, hepatobiliary, colorectal, sarcoma, brain, or breast cancer that may involve the axillary lymph nodes cancers. Tumors may be primary or metastatic
* Subject must understand the investigational nature of this study and sign an Independent Ethics Committee/Institutional Review Board approved written informed consent
* Subject must have a skin prick test pre-operatively (at the time of the preoperative visit and after signed informed consent for entry into this clinical trial is given) to determine any sensitivity to fluorescein

Exclusion Criteria:

* Uncontrolled intercurrent illness including, but not limited to, ongoing or active infection, symptomatic congestive heart failure, unstable angina pectoris, cardiac arrhythmia, or psychiatric illness/social situations
* Renal dysfunction as defined as a glomerular filtration rate (GFR) \< 45
* Liver dysfunction as defined by Child-Pugh score \> 5, or liver function test (LFT)'s 1.5 x above normal range
* Any known allergy or prior reaction to fluorescein or a positive skin prick test to fluorescein
* Pregnant or nursing female subjects, determined preoperatively with a urine pregnancy test
* Unwilling or unable to follow protocol requirements
* Any condition which in the investigators' opinion deems the patient unsuitable (e.g., abnormal electrocardiography \[EKG\], including T wave inversion, elevated T waves, prolonged QRS interval, or conduction blocks) or that requires further work-up (including cardiac echo or stress test)
* Any condition that excludes surgical resection as the standard of care for the patient